11/29/2023 0 Comments Excel scaffold albuquerque![]() This community-based action research examined the needs, problems, and local assets in the rural community of Magdalena, New Mexico. In rural communities, needs assessments can help prioritize issues/needs/wants among the various community stakeholders and inform comprehensive planning, program and policy development, and allocation of limited resources. Assessment data helps to inform the development of useful interventions and data informed decision-making processes that can later be evaluated for their effectiveness and/or to make improvements and adjustments to public policies, and local initiatives to reach community goals. Needs assessments help identify gaps in public policies and/or available public services, such as public utilities, public safety, education, housing, health, and transportation, as well as asses the local/regional economy that affect the quality of life of residents. Understanding community perspectives regarding local needs, problems and assets is an important step towards community development. The E-spin printer’s versatility with materials of disparate viscosities enabled the layer-by-layer fabrication of composite (PCL/PEGDA+bone) scaffolds that begin to mimic the complex nature of the bone-ligament interface.Ī Rural New Mexico Village's Perspectives of Local Problems, Strengths and Solutions: Asset Mapping and Issue Prioritization for Community Development The PCL fibers demonstrated a tensile strength approaching native ligament (3.96 ± 1.10 MPa), which shows promise as the ligament phase of the scaffold. ![]() Mechanical results suggest that the composite scaffolds have increased compressive strength over PEGDA alone as the PCL fibers constrict horizontal elongation, thus yielding a higher compressive modulus. Increased adhesion and spreading on decellularized bone scaffolds along with cell elongation in the direction of the fibers suggests the ability of the scaffold to encourage osteoblastic differentiation and ligamentous tissue formation, though a longitudinal study is still underway. The materials and the composite scaffold were seeded with 3T3 cells and cultured for three days before undergoing an immunocytochemistry staining (ICC) to assess cell adhesion and spreading. Cytotoxicity of all materials was determined through a Live/Dead assay (Thermo Fisher) with NIH/3T3 cells. ![]() Poly(ethylene glycol) diacrylate with decellularized bone was encased in the PCL fibers to create bony ligament support structures in a composite scaffold. We created NFE poly(caprolactone) highly aligned micro-fibers which formed collagen fibril-like bundles. This complex region is difficult to simulate due to its functionally graded mechanical and biochemical properties. We built a custom E-spin printer that enables layer-by-layer alternating deposition between 3D bioprinting and NFE to create composite scaffolds for the bone-ligament interface. Near-field electrospinning (NFE) offers precise alignment control to create non-woven mats with high tensile strengths. 3D bioprinting is an additive manufacturing technique that can utilize a range of bioactive materials to construct specific architectures that mimic native tissue.
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